HPLC Columns

C18 Analytical Column

Column Dimensions & Particle Size

The C18 Analytical Column is a reverse-phase HPLC column commonly used in pharmaceutical QC and method development. Typical dimensions include 250mm length and 4.6mm internal diameter with 3–5 µm particle size options, providing a balance of resolution and backpressure for routine assays. Selection focuses on column dimensions, particle size, and phase bonding to meet assay throughput and resolution requirements. Procurement teams choose C18 columns to standardize analytical runs across QC laboratories and method transfer programs.

Stationary Phase & pH Stability

C18 bonded silica stationary phase supports a wide range of reverse-phase applications. Buyers evaluate pH range compatibility and endcapping options for method robustness. The column suits laboratories performing impurity profiling, assay quantitation, and stability-indicating methods where reproducible retention and separation efficiency are required.